Mouse Connexin 43 ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Connexin 43 in biofluid samples, such as tissue homogenates, cell lysates and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . CX43 present in samples reacts with an anti-Mouse CX43 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-CX43 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active CX43 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse CX43. Detection Target: Mouse Connexin 43 Reactivity: Mouse Range: 0.156-10ng/mL Sensitivity: 0.052ng/mL Storage Conditions: Store all components at 4┬░C This Mouse CX43 activity assay is for the quantitative determination of Connexin 43 in samples including tissue homogenates, cell lysates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.